Investigation of the polyacrylamide - polyvinylimidazole - pentacyanoferrate(III) redox polymer as the electron mediator in the enzymatic assays

Abstract

The redox polymers are used in the enzymatic assays such as biosensors. During the research, the polyacrylamide - polyvinylimidazole - pentacyanoferrate(III) redox polymer was constructed. The optimum construction methods, also the stability were investigated. The acrylamide - vinylimidazole ratio in the polymer was determined. The spectrophotometric and voltamperometric measurements were used. The research showed that the modification of acrylamide side chains with hydrazine knocks out the pentacyanoferrate(III) out of the polymer.

Redoks polimerai yra naudojami elektrocheminėse fermentinėse sistemose tokiuose kaip biojutikliai. Šio tyrimo metu buvo sukonstruotas poliakrilamido-polivinilimidazolo- pentacianoferato(lll) redoks polimeras, optimizuoti sintezės metodai, ištirtas atsparumas destrukciniams veiksniams, nustatytas (poli)akrilamido ir (poli)vinilimidazolo santykis polimere. Tyrimai parodė, kad polimero modifikacija hidrazinu išmeta redoks aktyvius centrus iš polimero grandinių.